Indwara ya Marek Virus Antigen

Ibisobanuro

Indwara ya Marek Virus Antigen

Gukoresha: Kubwubushobozi bufatika bwa virusi ya Marek Antigen (MDV AG) muri serumu yinyoni, plasma cyangwa imyenda ya lesion .

Ihame: Urugwiro rutemba Imyunochromatograchic assay .

Ingero: Serum yinyoni, plasma cyangwa imyenda yabasimba

Ibikoresho byatanzwe:

Ibizamini 10, hamwe namakarita nabatonyanga

10 vial ya assay buffer

1 Ipaki

Ibiranga bidasanzwe:

1. sennsititivite yo hejuru kandi inyangamugayo .

2. inzira imwe yo kugerageza

3. ibisubizo nyabyo

Uburyo bw'ikizamini

-Ibisobanuro byinyoni cyangwa plasma mubice bya centrifuge . Menya neza ko icyitegererezo gisukuye. {2} {

-Niba icyitegererezo ni imyenda yo gutuza, gukusanya igice cyigice kinini muri issury buffer . no gukora tissue iboneka mugusaza buffer .

Koresha imvange ndengakamere mumashuri .

-Komera igikoresho cyibizamini kuva kumufuka wa Foil hanyuma ushire horizontally .

-Kongera gukuramo kwisumbabukuru kuva assay buffer tuffe hanyuma ushireho ibitonyanga 3 mucyitegererezo "s" ikizamini cyibizamini .

-Icyiza ibisubizo muri 5-10 iminota . ibisubizo nyuma yiminota 10 bifatwa nka .

Gusobanura ibisubizo

Ibyiza (+): Kubaho kw '"C" byombi hamwe na zone "t", nta murongo t usobanutse cyangwa udasobanutse.

Bibi (-): gusiba gusa C umurongo ugaragara . oya umurongo .

Bitemewe: Nta murongo wamabara ugaragara muri c zone . Ntakibazo niba t umurongo ugaragara .

Gutumiza amakuru

Ibirangantego: Indwara ya Marek Antigen, Ubushinwa Marek ya Virusi ya Virusi yo kurwanya ibizamini, abakora, uruganda