Indwara ya Bovine Rhinotracheitis Virus Antibody ELISA Igikoresho cyo Kumenya

Icyitegererezo No: VD105E
Abbr.:IBRV Ab Elisa
Icyitegererezo: serumu

Ibisobanuro

Ibisobanuro

BVDV Ab Elisa

Gukoresha: YanduyeIndwara ya Bovine RhinotracheitisAntibody ELISA Detection Kit yamenyereyemenya rhinotracheitis yanduyeantibodies za virusi muri serumu ya bovine muri vitro.

Ingero: serumu

Ibikoresho byatanzwe:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Uburyo bwo Kwipimisha & Gusobanura

INTAMBWE 1 Uburyo bwo Kwipimisha

1.1 Gukaraba igisubizo

Working kwibanda wkumesa amazi:Mbere yo gukoresha, igisubizo cyo gukaraba kigomba gusubizwa ubushyuhe bwicyumba (dogere 20-25) hanyuma kikanyeganyezwa kugirango kibe kimwe. Noneho shyira inshuro 20 igisubizo cyogejwe hamwe namazi meza (urugero, 5ml yumuti wogejwe wongeyeho amazi 95ml), vanga neza, kandi amazi yogejwe ashobora kubikwa kuri dogere 2-8 muminsi 7.

1.2 Icyitegererezo

Blood Amaraso yinyamaswa yose yarafashwe, ashyirwa muri 4000rpm muminota 10, hanyuma aregeranya ndengakamere. Serumu irashobora kandi gukonjeshwa bisanzwe nta hemolysis.

Kuramo serumu kugirango igeragezwe inshuro 50 muri plaque ya serumu (urugero: ongeramo 3ul ya serumu kugirango ugerageze kuri 147ul sample diluent).

Icyitonderwa: Igenzura ribi kandi ryiza ntirigomba gucibwa

1.3 Uburyo bwo Kwipimisha

AkeFata label ya enzyme Microplate (ukurikije umubare wintangarugero, irashobora gusenywa no gukoreshwa inshuro nyinshi), ongeramo 100ul ya serumu ivanze kuri enzyme label ya Microplate, shyira amariba abiri buriwese kugirango ugenzure nabi kandi ugenzure neza, hanyuma wongere 100ul mubitekerezo neza. Kunyeganyeza witonze icyitegererezo mu iriba, ukoresheje firime ya kashe kugirango utwikire isahani, hanyuma ushire kuri dogere 37 muminota 30.

②Gufungura icyapa gifunga kashe, ukureho igisubizo mu isahani neza, ongeramo 300µl yumurimo wo gukaraba wogeje amazi kuri buri riba, hanyuma ujugunye amazi mumiriba. Subiramo intambwe zavuzwe haruguru inshuro 5, hanyuma urangize gukama kumpapuro zisukuye.

Imisemburo ya enzyme ya conjugate: Koresha enzyme conjugate (80 ×) inshuro 80 (urugero: 10ul enzyme conjugate concentrate + 790 ul sample diluent, umubare wihariye usabwa ubarwa ukurikije umubare wibizamini), vanga neza hanyuma ushire kuruhande.

Ongeraho 100µl ya Enzyme Ihuze kuri buri riba, upfundike isahani, hanyuma ushire kuri dogere 37 muminota 30.

AsKwoza isahani ya enzyme, imikorere yihariye nimwe nkuko byavuzwe haruguru ②.

⑥ Ongeraho 100µl substrate igisubizo kuri buri riba, upfundike isahani hanyuma ushiremo iminota 10 kuri dogere 37 uvuye kumucyo.

INTAMBWE 2 Ibisobanuro byo gusobanura

Agaciro ka OD450 kuri buri riba ryasomwe kuri 96-iriba rya microplate.

2.1 Kwemeza Impuzandengo ya OD450nm yo kugenzura nabi yari <0.25 naho impuzandengo ya OD450nm yo kugenzura neza yari ikomeye kuruta cyangwa ingana na 0,6, ibisubizo byubushakashatsi byari bifite ishingiro. Bitabaye ibyo, ikizamini kigomba gusubirwamo.

2.2 Uburyo bwo Kubara

Kuri buri cyitegererezo, bara S / P kuburyo bukurikira ukoresheje icyitegererezo cyakosowe no kugenzura indangagaciro:

product-554-49

2.3 Ibisobanuro

Iyo S / P <0.2, bifatwa nkibibi.

Iyo S / P> 0.3, bifatwa nkibyiza.

Iyo 0.2 Ntibiri munsi cyangwa bingana na S / P Bitarenze cyangwa bingana na 0.3, bifatwa nkibikekwa, kandi bigasaba gusubiramo icyitegererezo no kugerageza nyuma yicyumweru.

Ibirangantego: virusi yanduye bovine rhinotracheitis virusi antibody elisa detection kit, Ubushinwa bwanduye bovine rhinotracheitis virusi antibody elisa detection ibikoresho bitanga ibikoresho, ababikora, uruganda

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