Ikirenge n'umunwa Virusi - o antibody Elisa Kit

Ibisobanuro

FMDV - o ab Elisa

Gukoresha: Ibirenge byo mu kanwa n'umunwa virusi - o

Antibody Elisa Kumenya Atrations ni uw'ibirenge n'umunwa antibod muri serumu ya Bovine muri Vitro. Ikizamini gikoreshwa mu gusuzuma ubwoko bwa virusi n'umunwa o kugabanya ubudahangarwa bw'imirima.

Ingero: Serumu

Ibikoresho birasabwa ariko ntibitangwa:

Amazi meza

Pipettor

Umusomyi wa Microplate 96 (450nm)

Igihe

Centrifuge (4000rpm)

Imashini imesa

Ifuru (Impamyabumenyi 37)

Uburyo bwo kwipimisha & gusobanura

Intambwe 1 IBIKORWA

1.1 Kama igiteguro cyo kwitegura

Working kwibanda wAshishing y'amazi:Mbere yo gukoresha, igisubizo cyo gukaraba kigomba gusubizwa mubushyuhe bwicyumba (impamyabumenyi 20-25) no kunyeganyeza cyane kugirango bibe umwe. Noneho dilute inshuro 20 Igiti cyibanze hamwe namazi meza (urugero, 5ml yibanda ku mazi 95ML yasukuye), kuvanga neza amazi meza, hamwe namazi yometseho arashobora kubikwa mu minsi 2-8.

1.2 Icyitegererezo cyo Kwitegura

Amaraso yose yinyamanswa yafashwe, arwanira kuri 4000rpm muminota 10, kandi ndengatse yakusanyijwe. Serumu irashobora kandi guhuzwa bisanzwe nta hemosise.

② Didute Omple 1:25 (Ongeramo 5ul icyitegererezo kuri 120ul icyitegererezo cya 120ul SamUnt) hanyuma uvange neza.

Icyitonderwa: Igenzura ribi kandi ryiza rikeneye na dilute.

1.3 Enzyme Kwitegura

Kuramo enzyme conjugate 1:90 (ongeramo 5ul enzyme conjugate (x100) kugeza kuri 4455 domple dilunt) hanyuma uvange neza.

1.4 Uburyo bw'ikizamini

① Fata microplate ya enzyme (ukurikije umubare wingengo yicyitegererezo, irashobora gucika intege inshuro nyinshi), ongeraho 100ul ya seriveri mbi ya enzyme, hanyuma ongeraho 100ul buriwese kubikorwa bibi kandi byiza kuri reaction. Shyira witonze icyitegererezo mu iriba, ukoresheje film yerekana ikiganiro kugirango utwikire isahani, kandi ushishikarire kurwego rwa 37 muminota 30.

②urangije firime y'ibipamba, uzunguze igisubizo kiri mu isahani, ongeramo 300μl yo gukaraba amazi kuri buri ndunduro, hanyuma ujugunye amazi mu iriba. Subiramo intambwe zavuzwe haruguru, hanyuma urangize wumye impapuro zikurura.

③add 100μl ya enzyme guhuza kuri buri ndaba, twimbaze isahani, kandi ushiremo impamyabumenyi 37 muminota 45.

④urangije firime y'ibipamba, uzunguze igisubizo kiri mu isahani, ongeramo 300μl yo gukaraba amazi kuri buri ndunduro, hanyuma ujugunye amazi mu iriba. Subiramo intambwe zavuzwe haruguru, hanyuma urangize wumye impapuro zikurura.

⑤add 100μl Restaut Igisubizo kuri buri cyenda, gitwikire isahani kandi iminota 10 kuri leta 37 kure yumucyo.

⑥add 50μl guhagarika igisubizo kuri buri jambo, kandi umenye ibisubizo muminota 5.

Intambwe 2 Ibisubizo Ibisobanuro

Agaciro ka OD450 ya buri jambo ryasomwe kubasomyi 96-neza.

2.1 Kwemeza

Impuzandengo ya Od450nm Agaciro ko kugenzura nabi byari<0.15 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥3, the experimental results were valid. Otherwise, the test should be repeated.

2.2 Uburyo bwo kubara

Kuri buri cyitegererezo, kubara s / p nkibi bikurikira ukoresheje icyitegererezo cyakosowe hamwe nindangagaciro zigenzura:

2.3 Gusobanura

Iyo s / p<0.15, it is judged as negative.

Iyo S / P irenze cyangwa ingana na 0.25, ifatwa nkibyiza.

Iyo 0.15 munsi cyangwa ingana na s / p<0.25, it is judged as suspicious, and suggest to repeat sampling and test one week later.

Ibirangantego: Ikirenge n'umunwa Virusi - O Antibody Elisa Kit, Ubushinwa Ikirenge n'umunwa virusi - o antibods Abatanga ibikoresho, abakora, uruganda