Brucella antimadlisa Kit
Model No: VD503E
Abbr .: bru ab Elisa
Spemiten: Serum
Ibisobanuro
Ibisobanuro
Gukoresha: Ikizamini gikoreshwa mu kumenya antibodies kurwanya Brucella Abortus (inka) na Brucella Maltese (intama n'ihene) .
Ingero: Serumu
Ibikoresho birasabwa ariko ntibitangwa:
Uburyo bwo kwipimisha & gusobanura
Intambwe 1 IBIKORWA
1.1 Kama igiteguro cyo kwitegura
Working kwibanda wAshishing y'amazi:Mbere yo gukoreshwa, igisubizo cyo gukaraba kigomba gusubizwa mubushyuhe bwicyumba ({{0} iminsi .
1.2 Icyitegererezo cyo Kwitegura
Amaraso yinyamanswa yafashwe, ashonga kuri 4000rpm muminota 10, kandi ndengakamwe yakusanyijwe .}}}
②Iyo ingano yintangarugero nini, kugirango hakemure igihe cyimyitwarire ihamye . ibyuma bifatika hamwe nisahani nziza ni kimwe na serum icyitegererezo .
1.3 Uburyo bw'ikizamini
① Fata microplate ya enzyme (ukurikije umubare wingengo yicyitegererezo, irashobora gucika intege kandi igashyiraho amayeri ya enzyme kuri enzyme label, akoresheje firime yinjira neza. iminota .
②urangije firime ya salation, kuramo igisubizo mu isahani neza, ongeramo 300μl yo gukaraba amazi yavuzwe muri buri . subiramo impapuro zo hejuru .
③Divage enzyme conjugate:Dilute enzyme conjugate (50 ×) inshuro 50 (Urugero: 20ul enzyme guhuza wibanda + 980 ul diluntion 1, kuvanga neza no gushiraho neza hanyuma ushireho kuruhande .
④add 100μl ya enzyme guhuza kuri buri ndaba, fungura isahani, kandi ushishikarire kurwego rwa 37 muminota 30 .
⑤kushing enzyme plate, igikorwa cyihariye ni kimwe no hejuru ② {{0}
Yamazaki
⑦add 50μl guhagarika igisubizo kuri buri cyenda, kandi umenye ibisubizo muminota 5 .
Intambwe 2 Ibisubizo Ibisobanuro
Agaciro ka OD450 ka burikuri byasomwe kuri 96- icyuma cyiza microplate umusomyi .
2.1 Kwemeza
Impuzandengo ya Od450nm Agaciro ko kugenzura neza yari <0. 35 hamwe na od450nm agaciro kanini / kugenzura icyiza cyari gifite ishingiro. Bitabaye ibyo, ikizamini kigomba gusubirwamo.
2.2 Uburyo bwo kubara
Kuri buri cyitegererezo, kubara Pi (Blokinr yariye) kuburyo bukurikira ukoresheje icyitegererezo cyakosowe hamwe nindangagaciro zigenzura:
2.3 Gusobanura
Iyo PI <20%, ifatwa nkibibi .
Iyo PI> 30%, ifatwa nkibyiza .
Iyo 20% munsi cyangwa bingana na pi munsi cyangwa bingana na 30%, bifatwa nkibiteye inkeke, kandi bikagira inama yo gusubiramo icyiciro no kugerageza icyumweru kimwe nyuma yicyumweru .
Ibirangantego: Brucella antimay Elisa Kit, Ubushinwa Brucella Antindy Elisa Abatanga ibikoresho, abakora, uruganda
